个人信息

龙梦飞，男，汉族，中共党员。本科毕业于西南大学制药工程专业，博士毕业于江南大学发酵工程专业。主持国家重点研发计划“合成生物学”重点专项子课题、国家自然科学基金青年项目、中国博士后科学基金、重庆市自然科学基金面上项目、江苏省青年科学基金、重点实验室开放课题及企业委托课题等项目5项；在Sci Adv, ACS Catal, ACS Synth Biol, Bioresour Technol, Food Hydrocolloid, Crit Rev Food Sci Nutr, J Agric Food Chem, Food Biosci, Appl Environ Microbiol, Appl Microbiol Biotechnol,《中国科学：生命科学》及《生物工程学报》等领域权威期刊上发表论文30篇；申请国家发明专利15件，授权7件，参编论著及教材3部；参与制修订行业/团体标准6件；获中国食品科学技术学会二等奖、中国商业联合会二等奖等奖项5项。

研究方向与兴趣

利用合成生物学、计算生物学等手段探究细胞内蛋白质/酶的生物合成-折叠、功能-调控及质量控制机制，并开发特色酶工程及细胞改造策略，实现药物及其中间体、高值化学品等高效生物制造。

教育及工作经历

2011.09-2015.09，西南大学药学院，制药工程学士

2015.09-2021.06，江南大学生物工程学院，发酵工程博士

2021.07-2023.05，江南大学生物工程学院，助理研究员

2023.06-至今，西南大学药学院，讲师

主持科研项目
1.“绿色生物制造”国家重点研发计划子课题，2023.01-2027.122.
2.国家自然科学基金青年项目，2025.1-2027.12，
3.中国博士后科学基金，2023.01-2025.12
4.江苏省青年科学基金，2022.07-2025.06
5.重庆市自然科学基金面上项目，2025.06-2028.06
6.重庆市重点实验室开放基金，2025.01-2026.12
7.中央高校基本科研基金，2022.01-2023.12

代表论文

1. Mengfei Long #, Meijuan Xu #, Zhenfeng Ma, Xuewei Pan, Jiajia You, Mengkai Hu, Yu Shao, Taowei Yang, Xian Zhang, Zhiming Rao*. Significantly enhancing production of trans-4-hydroxy-L-proline by integrated system engineering in Escherichia coli. Science Advances, 2020, 6(21):eaba2383.

2. Mengfei Long, Meijuan Xu*, Zhina Qiao, Zhenfeng Ma, Tolbert Osire, Taowei Yang, Xian Zhang, Minglong Shao, Zhiming Rao*. Directed evolution of ornithine cyclodeaminase using an EvolvR-based growth-coupling strategy for efficient biosynthesis of L-proline. ACS Synthetic Biology, 2020, 9(7):1855-1863.

3. Mengfei Long#, Yongchao Cai#, Nan Zheng, Zhi Lu, Wentao Cao, Yaxian Li, Xiaomei Pei, Osire Tolbert, Xiaole Xia*. Clean Monascus pigments production from Chinese rice wine wastes through submerged fermentation. Food Bioscience, 2023,52:102451.

4. Guojuan Yi#, Hanlu Zou#, Tao Long, Tolbert Osire, Lin Wang, Xiaoyun Wei, Mengfei Long*, Zhiming Rao, Guojian Liao*. Novel cytochrome P450s for various hydroxylation of steroids from filamentous fungi. Bioresource Technology, 2023, 130244.

5. Mengfei Long, Xiaomei Pei, Zhi Lu, Duo Xu, Nan Zheng, Yaxian Li, Hanxiao Ge, Wentao Cao, Tolbert Osire, Xiaole Xia*. Effective degradation of anthraquinones in Folium Sennae with Monascus fermentation for toxicity reduce and efficacy enhancement. Heliyon, 2023, 9(8).  

6. Zidan Liu, Weihao Long, Keying Chen, Linyu Luo, Qiong Li, Tolbert Osire, Nan Zheng*, Mengfei Long*. Function and engineering of a food enzyme under coupled high-temperatur-pressure conditions: insights from molecular dynamics simulation and experimental validation. Foods, 2025, 14(14): 2485.

7. Zidan Liu, Linyu Luo, Zhiqiang Ding, Weihao Long, Tolbert Osire, Qiong Li,Qianfeng Chen, Mengfei Long*. Ultrasound-assisted coupled with resin-based purification for sustainable extraction of steviosides from Stevia rebaudiana leaves. Molecules, 2025, 30(16): 3416.

8. Huan Long, Zhongjie Zeng, Tolbert Osire, Jiang Zhu*, Mengfei Long*. in vitro  and in silico  analysis reveal the potential mechanisms of Liu-Wei-Di-Huang-Wan formula for tinnitus treatment. Letters in Drug Design & Discovery, 2025. (accepted)

9. Jiadian Wang, Qin Xie, Xinmeng Wang, Mengfei Long, Yanying Chen, Zheng Liu, Meng Xia, Juan Guo, Zeping Wang, Rongfeng Wang, Siyu Shen, Yun Lu, Yan Yin, Yating Hu, Wei Gao*, Xiao Zhang*, Ping Su*, Luqi Huang*. Discovery of key cytochrome P450 monooxygenase (C20ox) enables the complete synthesis of tripterifordin and neotripterifordin. ACS Catalysis, 2025, 15: 2690-2702.

10. Peifeng Duan#, Mengfei Long#, Xian Zhang, Zuyi Liu, Jiajia You, Xuewei Pan, Weilai Fu, Meijuan Xu, Taowei Yang, Minglong Shao, Zhiming Rao*. Efficient 2-O-α-D-glucopyranosyl-sn-glycerol production by single whole-cell biotransformation through combined engineering and expression regulation with novel sucrose phosphorylase from Leuconostoc mesenteroides ATCC 8293. Bioresource Technology, 2023, 129399.

11. Zehua Zhang#, Mengfei Long#, Nan Zheng, Yu Deng, Qiong Wang, Tolbert Osire, Xiaole Xia*. Microstructural, physicochemical properties, and interaction mechanism of hydrogel nanoparticles modified by high catalytic activity transglutaminase crosslinking. Food Hydrocolloids, 2024, 147, part A:109384.

12. Nan Zheng#, Mengfei Long#, Zehua Zhang, Qijia Zan, Tolbert Osire, Huimin Zhou, Shuang Du, Xiaole Xia*. Functional, structural properties and interaction mechanism of soy protein isolate nanoparticles modified by high-performance protein-glutaminase. Food Hydrocolloids, 2022,139:108594.

13. Nan Zheng#, Mengfei Long#, Zehua Zhang, Shuang Du, Xinlei Huang, Tolbert Osire, Xiaole Xia*. Behavior of enzymes under high pressure in food processing: mechanisms, applications, and developments. Critical Reviews in Food Science and Nutrition, 2023,1-15.

14. Nan Zheng#, Mengfei Long#, Zehua Zhang, Qijia Zan, Tolbert Osire, Huimin Zhou, Xiaole Xia*. Protein-glutaminase engineering based on isothermal compressibility perturbation for enhanced modification of soy protein isolate. Journal of Agricultural and Food Chemistry, 2022, 70(43), 13969–13978. (Cover story)

15. Zehua Zhang#, Mengfei Long#, Nan Zheng, Xiang Lv, Cailin Zhu, Tolbert Osire, Xiaole Xia*. Inside-out computational redesign of cavities for improving thermostability and catalytic activity of Rhizomucor miehei Lipase. Applied and Environmental Microbiology, 2023, e02172-22.

16. Zehua Zhang#, Mengfei Long#, Nan Zheng, Yu Deng, Qiong Wang, Tolbert Osire, Xiaole Xia. Redesign of γ-glutamyl transpeptidase from Bacillus subtilis for high-level production of L-theanine by cavity topology engineering. Applied Microbiology and Biotechnology, 2023, 107, 3551-3564.

17. Qijia Zan#, Mengfei Long#, Nan Zheng, Zehua Zhang, Huimin Zhou, Xinjie Xu, Tolbert Osire, Xiaole Xia*.  Improving ethanol tolerance of ethyl carbamate hydrolase by diphasic high pressure molecular dynamic simulations. AMB Express. 2023. 13, 32.

18. Xue Dong#, Mengfei Long#, Hongxia Liu, Ling Gao, Xinjie Xu, Xiaole Xia*. Synthesis and characterization of eco-friendly poly(ε-caprolactone) plasticizer facilitating phthalate-free PVC with novel star/net-shaped structures. Journal of Applied Polymer Science, 2023, e53905. (Cover story)

19. Xue Dong#, Mengfei Long#, Yongchao Cai, Zhemin Zhou*, Xiaole Xia*. Novel biologically derived tartaric acid plasticizer: Synthesis and application. Journal of Applied Polymer Science, 2023, e54707.

20. 龙梦飞，徐美娟，张显，杨套伟，邵明龙，许正宏，饶志明*. 合成生物学与代谢工程在谷氨酸棒杆菌产氨基酸中的应用. 中国科学•生命科学, 2019, 49: 1-12

21. 龙梦飞#，郑楠#，张泽华，高玲，王颖妤，夏小乐*. 蛋白质交联用酶的作用机制及研究进展. 生物工程学报, 2022, 38(7): 2499-2512.

(#equal contribution；*corresponding author)

22. 刘子丹, 隆伟豪, 杜红毅, 陈柯颖, 邱钦亮, 龙梦飞*.多铜氧化酶在生物胺降解中的应用进展与分子改造策略.中国生物工程杂志, 2025, 1-10.

获奖情况（含指导学生获奖）
1. 中国商业联合会科技进步二等奖（2023）
2. 中国食品科学技术学会技术进步二等奖（2022）
3. “互联网+”大学生创新创业大赛全国铜奖（2017，2021）
4. “创青春”中国青年碳中和创新大赛省级银奖（2025）

邮箱：mengfeilong@swu.edu.cn

地址：重庆市北碚区天生路2号西南大学药学院400715

Talk is cheap, show me your thinking, coding and wet/dry validating.